Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

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We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets.The results showed that skull bride and groom both A-cells (glucagon-secreting cells) and PP-cells (PPsecreting cells) were located in the periphery of the islets.However, A-cells and PP-cells had a different regional distribution.Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas.

In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of opi the color that keeps on giving the pancreas.Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining.

Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells.